Phenolic Characterization and in vitro Biological Activities of Ranunculus Cornutus DC.

Abstract

In this study, Ranunculus cornutus DC. (Evlimemedotu) content analysis and some biological activities were investigated. For this purpose, methanol and water extracts of the plant were taken and their phenolic analyzes were determined by LC-MS-MS. Total antioxidant method, FRAP, CUPRAC, iron chelation method, DPPH and ABTS methods were used for antioxidant studies. According to LC-MS-MS results, the highest amounts of phenolic substances were determined as fumaric acid (3261.43 µg/L), vanillic acid (1217.70 µg/L) and ferulic acid (769.40 µg/L), respectively. According to the antioxidant results, it was determined that water and methanol extracts exhibited lower activity than standard antioxidants. It can be said that the reason for this is the amount of phenolic they contain. However, it was observed that the methanol extract generally had better antioxidant activity than the water extract. Finally, according to the DNA study results, it was determined that water and methanol extracts alone did not have a negative effect on the stable structure of DNA. Since there are no studies with R. cornutus DC., it is thought that this study will make a very important contribution to the literature and provide important data for future studies.