COMPETITIVE INHIBITION OF GLUTATHIONE REDUCTASE ACTIVITY BY CYCLOHEXYL-(4,5-DIHYDRO1H-IMIDAZOL-2-YL)AMINE HYDROIODIDE AND BENZYL(4,5-DIHYDRO-1H-IMIDAZOL-2-YL)AMINE HYDROIODIDE

Abstract

In this research, 0, 25, 50, 100, 200 and 400 mg/L (ppm) concentrations of 2aminoimidazolines (cyclohexyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide (CHX) and benzyl-(4,5-dihydro-1H-imidazol-2-yl)amine hydroiodide (BNZ)) were applied to baker's yeast (Saccharomyces cerevisiae) glutathione reductase (GR). Since the inhibition constant (Ki) (43 ± 2 nM) and 50 % inhibition concentration (IC50) (947 mg/L =3.13×10-3 M) values of BNZ were found to be smaller than the Ki (59 ± 17 nM) and IC50 (1461 mg/L =4.95×10-3 M) values of CHX. At imidazoline concentrations of 400 mg/L, CHX reduced GR activity by 16.07 %, while BNZ reduced GR activity by 21.96 %. BNZ inhibited GR more than CHX. In addition, molecular docking results also support the experimental data. CHX and BNZ inhibited GR enzyme activity as competitively.