A Hydrophillic Deep Eutectic Solvent Assisted Magnetic Colloidal Gel Based Dispersive Solid Phase Microextraction Method for Preconcentration of Brown HT (E155)

Abstract

A hydrophillic deep eutectic solvent assisted magnetic colloidal gel based dispersive solid phase microextraction method (MCG-dSPME) was developed for the pre-concentration of Brown HT (E155) before its spectrophotometric analysis. Magnetic colloidal gel was obtained from Fe3O4@XAD-7 nanoparticles and phenol/choline chloride (2:1) (hyrophillic deep eutectic solvent (DES). For the optimization of the developed method, several parameters such as pH, amount of Fe3O4@XAD-7 nanoparticles in the colloidal gel, volume of colloidal gel, type of desorption solvent, vortex time (for adsorption and desorption), sample volume were investigated. After determining the optimum conditions, the linear range, limit of detection (LOD), enhencament factor (EF), preconcentration factor (PF), relative standard deviation (RSD %) of the method were calculated. Then, addition/recovery test and intraday-interday test were applied for the accuracy and the precision, respectively. The matrix effect study was examined for the selectivity of the method. The linear range, LOD, RSD %, EF, and PF values of the method were found to be 0.05-0.75 mgL-1, 0.016. mgL-1, 3.8%, 51 and 25, respectively. The study was found to be no interference method with high accuracy and precision. In this study, according to our literature research, since there are very few studies on the determination of Brown HT, it was aimed to develop an up-to-date, economical, non-invasive, environmentally friendly and simple method for this dye.