Show simple item record

dc.contributor.authorAlkış, Mehmet Eşref
dc.contributor.authorALAN, Yusuf
dc.contributor.authorESER, Erhan
dc.date.accessioned2025-08-13T07:46:10Z
dc.date.available2025-08-13T07:46:10Z
dc.date.issued2024
dc.identifier.issn2147-3129
dc.identifier.urihttp://dspace.beu.edu.tr:8080/xmlui/handle/123456789/15660
dc.description.abstractElectroporation (EP) is the process of increasing the permeability of a biological cell or tissue by applying a short-term and sufficient external electric field. The utilization of proper pulse settings is required for EP-based treatments to be successful. Our aim in this study was to examine the effect of different electrical pulse widths and strength on EP efficiency. Human osteosarcoma cells (U2OS) were used in the study. Eightsquare-pulses with a frequency of 1Hz at 10µs, 1ms, 5ms, 10ms, and 20ms widths with low electric fields (20-500V/cm) were applied to U2OS cells. 10-15 minutes after the applications, the cells were incubated in 96-well plates with 10 thousand cells in each well for 24 hours. Efficiency of pulses of different intensity and width was evaluated by MTT analysis method. The percent inhibition of U2OS cancer cells elevated as the pulse width increased in almost all electric field values. The highest cell inhibition (%) occurred in pulses with an electric field of 500 V/cm and a width of 20ms (inhibition ratio: 76.25%). No inhibition was observed in the cells at 10µs, 1ms, 5ms, 10ms width pulses with 20 V/cm electric field and 10µs, 1ms width pulses with 50V/cm electric field. In conclusion, our findings show that the electric field intensity and pulse width used in electroporation play an important role in U2OS cancer cell death. According to our results, it may be more appropriate to use highvoltage short-width pulses or low-voltage long-width pulses in reversible EP studies.tr_TR
dc.language.isoEnglishtr_TR
dc.publisherBitlis Eren Üniversitesitr_TR
dc.rightsinfo:eu-repo/semantics/openAccesstr_TR
dc.subjectElectroporation,tr_TR
dc.subjectPulse Width,tr_TR
dc.subjectPulse Strength,tr_TR
dc.subjectOsteosarcoma,tr_TR
dc.subjectToxicitytr_TR
dc.titleEffect of Pulse Width and Intensity on Cell Death in Reversible Electroporation of Cancerous Cellstr_TR
dc.typeArticletr_TR
dc.identifier.issue3tr_TR
dc.relation.journalBitlis Eren Üniversitesi Fen Bilimleri Dergisitr_TR
dc.identifier.volume13tr_TR


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record