A Comparative Study of the Antiproliferative and Apoptotic Effects of Some Chemotherapeutic Drugs on Neuroblastoma Cells
Abstract
In this study, it was aimed to investigate the antiproliferative and apoptotic effects of
nivolumab, cetuximab and gemcitabine used in the treatment of different cancer
types as well as cisplatin and cyclophosphamide used in the treatment of
neuroblastoma on SH-SY5Y neuroblastoma cells. The effect of each
chemotherapeutic on cell viability and the individual half-maximal inhibitory
concentration (IC50) values were determined by the crystal violet method. To
determine their apoptotic effects, RT-PCR and Annexin V-FITC apoptosis detection
technique were used. The results indicated that all the used chemotherapeutic drugs
showed dose-dependent cytotoxic effects and induced apoptosis in SH-SY5Y cells.
The IC50 values of cisplatin, cyclophosphamide, nivolumab, cetuximab, and
gemcitabine were calculated as 10.91 µM, 0.54 µM, 30.26 μM 4.74 μM and 0.036
μM, respectively. After IC50 dose treatment of cisplatin, cyclophosphamide,
nivolumab, cetuximab, and gemcitabine, apoptotic cell rates were found as 21%,
12%, 16%, 10% and 39% respectively. It was determined that statistically significant
changes in mRNA expression levels in almost all apoptosis-related genes occurred
after chemotherapeutic drugs treatment. In conclusion, gemcitabine showed more
antiproliferative and apoptotic effects on neuroblastoma cells than the other
chemotherapeutics. It is clear that further studies that will elucidate the mechanism
of action of gemcitabine may contribute to the treatment of neuroblastoma
Collections
DSpace@BEU by Bitlis Eren University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..