Determination of MIC and MBC Values Using Different Extraction Methods in Plants of Nigella Sativa, Cuminum Cyminum and Pimpinella Anisum L. Samples from Kırıkkale Region
Abstract
The objective of our study was to investigate how 6 plant extracts derived from three plant species exhibit antimicrobial characteristics. The extraction process was conducted using methanol and employed both maceration and soxalet extraction methods. The liquid microdilution method was utilized to assess the ability of Black Cumin (Nigella sativa), Cumin (Cuminum cuminum), and Anise (Pimpinella anisum) cultivated in Kırıkkale province to combat Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). All of the extracts of Nigellla sativa (black cumin), Cuminum cuminum (Cuminum) and Pimpinella anisum (Anise) prepared by the maceration and soxalet extraction method showed antibacterial effects against both bacteria (inhibitory and bactericidal). it was determined that the antibacterial effect of the extracts obtained by the maceration method was higher than the extracts obtained by the soxalet extraction method
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