Molecular Typing with BOX-PCR of Staphylococcus aureus Isolated from Milk Samples

Abstract

The aim of this study is to characterize 98 Staphylococcus aureus isolated from bovine subclinical mastitis in Konya region with BOX primer. A total of 28 band profiles ranging from ~ 950-300 bp were generated by the BOX primer. This band profile was evaluated according to Nei homology and a dendogram (UPGMA) was obtained. According to the data obtained, 95 isolates with genetic similarity varying between 50-100% were in the same group. This study demonstrates that the BOX-PCR technique provides a fast and reliable tool to investigate epidemiology in S. aureus.