A Morphological and Stereological Study on Cervical Spinal Cord of One and Five Months Age Male Rat
Abstract
In this study volume density of gray and white matter of cervical segments of spinal cords of rats were investigated
using stereological method. Twelve male Wistar albino rats were used in the study as two different age groups 1
month and 5 months. All animals were fixed by perfusing 10% buffered formalin. Rats were dissected and spinal
cords of rats were removed. In the study cervical segments of 1 and 5 months age rats were obtained. One of the
first 15 section were selected randomly when the sections were taken. And following every 50th section was
determined by systematic random sampling. Thus, 8-10 sections of 5µm thickness were obtained from cervical
segments of each animal’s medulla spinalis. These sections were stained by hemotoxylin eosin and they were
photographed at microscope. Densities of volumes of all tissue of cervical segments of whole spinal cord and white
and gray matters were calculated using dotted area ruler by Cavalieri Principle. SHTEREOM 1.5 package
programme was used for counting dotted area. In addition, the volume vales of total cervical segment, volume
values of the white matter and the gray matter and the ratios of these volume values to each other were evaluated
in the study
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