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    • 03) Bitlis Eren Üniversitesi Fen Bilimleri Dergisi
    • Cilt 07, Sayı 1 (2018)
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    • 03) Bitlis Eren Üniversitesi Fen Bilimleri Dergisi
    • Cilt 07, Sayı 1 (2018)
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    Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes

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    Date
    2018
    Author
    KILINÇ, Namık
    ALIM, Zuhal
    ŞENGÜL, Bülent
    İŞGÖR, Mehmet Mustafa
    BEYDEMİR, Şükrü
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    Abstract
    Carbonic anhydrase enzyme plays a vital role in metabolic events such as acid-base regulation and respiration. In our research, it is tried to determine the inhibitory influences of the cardiovascular therapeutics esmolol hydrochloride, amiodarone hydrochloride and lidocaine hydrochloride on human erythrocytes carbonic anhydrases (hCA I and II). In accordance with this purpose, carbonic anhydrase isoenzymes were purified from human erythrocytes by using affinity chromatography method. Enzyme purity was checked by SDS-PAGE electrophoresis method. After the carbonic anhydrase enzymes were purified, the inhibitory affects of cardiovascular therapeutics on these enzymes, using esterase activity, which is the method of measuring in vitro activity, were examined. The three cardiovascular therapeutics dose-dependently decreased activity of hCAs. IC50 values of amiodarone hydrochloride, esmolol hydrochloride and lidocaine hydrochloride were found to be, respectively, 0.91 mM, 5 mM, 5.8 mM for hCA-I and 0.41 mM, 3.5 mM, and 6.36 mM for hCA-II. Our results proved that, under in vitro conditions, cardiovascular therapeutics significantly inhibit human CA-I and II activities. So, irregular use of these medicines may cause serious adverse effects in terms of human health.
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    http://dspace.beu.edu.tr:8080/xmlui/handle/123456789/14149
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