Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes
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Date
2018Author
KILINÇ, Namık
ALIM, Zuhal
ŞENGÜL, Bülent
İŞGÖR, Mehmet Mustafa
BEYDEMİR, Şükrü
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Carbonic anhydrase enzyme plays a vital role in metabolic events such as acid-base regulation and respiration. In
our research, it is tried to determine the inhibitory influences of the cardiovascular therapeutics esmolol
hydrochloride, amiodarone hydrochloride and lidocaine hydrochloride on human erythrocytes carbonic anhydrases
(hCA I and II). In accordance with this purpose, carbonic anhydrase isoenzymes were purified from human
erythrocytes by using affinity chromatography method. Enzyme purity was checked by SDS-PAGE
electrophoresis method. After the carbonic anhydrase enzymes were purified, the inhibitory affects of
cardiovascular therapeutics on these enzymes, using esterase activity, which is the method of measuring in vitro
activity, were examined. The three cardiovascular therapeutics dose-dependently decreased activity of hCAs. IC50
values of amiodarone hydrochloride, esmolol hydrochloride and lidocaine hydrochloride were found to be,
respectively, 0.91 mM, 5 mM, 5.8 mM for hCA-I and 0.41 mM, 3.5 mM, and 6.36 mM for hCA-II. Our results
proved that, under in vitro conditions, cardiovascular therapeutics significantly inhibit human CA-I and II
activities. So, irregular use of these medicines may cause serious adverse effects in terms of human health.
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